Genome sequence comparison of tumor and non-tumor samples obtained from brown bullhead fish from Presque Isle State Park was undertaken to identify regions of DNA mutations in tumor samples. DNA primer sets were constructed to amplify and examine eight of these regions in greater detail, all of which successfully amplified the region targeted. Three genome areas were targeted for sequence analysis in a greater subset of tumor and non-tumor fish samples. The sequence analysis of these samples indicated identical sequence similarity between the tumor and non-tumor samples for all genome areas targeted. The results indicate a non-uniform environmental DNA mutation profile explaining tumor formation in brown bullheads.