Invasive crustacean species disturb existing food webs, macrophyte, benthic invertebrate and fish populations, and negatively affect overall ecosystem function. Originally introduced through aquaculture, aquarium and pond trades, and as live bait, crustacean species have invaded aquatic habitats globally, outcompeting native species and depleting aquatic vegetation. The crayfish species Orconectes rusticus (Rusty crayfish) native to the mid-west, has invaded aquatic habitats throughout northeastern North America and Europe. In Pennsylvania, Rusty crayfish dominate the Susquehanna River and many of its tributaries, displacing native speciesThis project will investigate the potential utilization of species-specific pheromones to improve trapping efficiency and possibly create chemical barriers to the invasive crustacean species, Rusty crayfish. Studies and experiments will be completed in the field and laboratory by science faculty and students at Elizabethtown College. Existing aquatic quality and organisms in the Susquehanna River tributaries, Connoy Creek and the Conewago Creek, will be surveyed and crayfish identified to species based on morphology and DNA sequences. Pheromones will be evaluated that could elicit attractive or avoidance responses by crayfish species and that could possibly be used in management programs to reduce the invasive species populations.